spectramax i3x. Summary of Contents for Molecular Devices SpectraMax M2. spectramax i3x

 
Summary of Contents for Molecular Devices SpectraMax M2spectramax i3x  Pick the best speed, sensitivity, and price for your needs

The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 1. In brief, a pre-titrated dose of virus was incubated with 8 serial 5-fold dilutions of serum samples in duplicate in. After 2 hours of treatment cells were harvested by centrifugation and cell pellets were lysed in RIPA buffer (20mM Tris-Cl, 150mM NaCl, 0. infoboxeu@moldev. 2 Note Section Patch:1) Select the correct plate and the wells that you want to measure. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. The user guide also covers the features and specifications of the SpectraMax MiniMax 300. Shaking and incubations were performed using an on-deck orbital shaker. 100 fmol 384-well (75 µL) 0. For each kit, the reader was able to detect the lowest dilution as positive. FTIR Spectrometer: Shimadzu IRSpirit (2018)The plate was read on the SpectraMax i3x Multi-Mode Microplate Reader. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. 0´ 105 dsD NA (p g/ wel ) R F UFluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). Danaher Inc spectramax i3x. Get a front row view of your cells. Microplate Reader Shelf. STAKMAX. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. . Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Video: SpectraMax i3x Overview; Brochure: SpectraMax i3x Multi-Mode Detection PlatformVersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. ”Figure 3: Sensitivity of the SpectraMax i3x reader in detecting increasing dilutions of the MycoAlert Assay Control. SeeSpectraMax MiniMaxImagingCytometeronpage85. Genscript cPass SARS-CoV-2 Neutralization Antibody Detection. . HTRF IP standard curve. . 더 많은. ZERO BIAS - scores, article reviews, protocol conditions and more. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. SpectraMaxi3Multi. were measured at OD405 using a SpectraMax i3x multimode detection system (Molecular Devices, San Jose, CA, USA), and the melanin concentration was determined using the standard curve of melanin solutions with known concentrations. , Fisher Scienti fi c, cat. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. Upon completion of a plate read, data are delivered to your computer in an Excel-compatible format. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. The SpectraMax i3x reader provides the. Amplitude : 1. Winnersh Triangle. Note: Additional readers with similar performance are listed. Spectranet customer portalThe SpectraMax i3x reader and MiniMax cytometer can be used with FluoroBlok cell culture inserts to directly count cells from high-quality images to measure cellular migration over time. Description 1. Modularity does provide some measure of forward vision into upcoming or future experimental needs. 99. 020 fmol 0. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. 0´ 104 8. 2. View the Molecular Devices website. . Designed specifically for the SpectraMax® i3 Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick inspection of cells and provides insight into phenotypic changes that are important for understanding cytotoxicity, cell proliferation, and protein expression. Molecular Devices推出的SpectraMax® i3x 多功能酶标仪除了具有全波长的光吸收、荧光、化学发光和FRET检测功能以外,还兼容了客户端模块化升级功能,用户可以根据需要任何时间随意升级至荧光偏振FP、HTRF、AlphaScreen、Western Blot、上转发光检测、细胞成像和带有注射器. They include the SpectraMax® iD3 Multi-Mode Microplate Reader, SpectraMax® L Microplate Reader, SpectraMax® i3x Multi-Mode Microplate Reader, and SpectraMax® M5 Multi-Mode Microplate Reader. The calculated LOQ values were 0. HTRF IP standard curve. Controls containing all reaction. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Cet appareil ISM est confomre à la norme NMB-001 du Canada. SpectraMax i3x Multi-Mode Detection Platform. The SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer is equipped with transmitted light (TL), green fluorescence (Ex/Em: 460/541), and red (625/713) fluorescence channels. 5 Figures from 202 Publications Thermo Fisher Scientific. Subsequently, 100 μL of the substrate solution (2 mM of p-nitrophenyl butyrate in dimethylformamide) was added into the mixture, and incubated at 37°C for 15 min. EUROPE. SPECTRAMAX i3X, INJECTOR MODULE CARTRIDGE model number 0200-7029. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Read height should be optimized for . +44 118 944 8000. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. The SpectraMax Injector Cartridge enables precise reagent delivery and adjustment of delay and integration times that are required to obtain optimal sensitivity for this assay. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Sensitive TRF detection of two simple, mix-and-measure assay workflows. StakMax Handling Stacker Base Unit. Fluorescent Polarization: Solid black plates, as above. BMG LABTECH VANTAstar. Capture Flash Assays with Ease. . Expand your lab’s capabilities to include flash applications such as dual luciferase and. The SpectraMax i3x reader provides the The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax. The plate was then read on the SpectraMax iD5, SpectraMax i3x, and SpectraMax M5e readers using the settings in Table 1. After 2 days of incubation, LUC and REN activities were measured using a SpectraMax ® i3x Multi-Mode detection platform (Molecular Devices, USA) with a Dual-Luciferase Reporter Assay Kit (Promega. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. The StemoniX microBrain 3D platform is a homogenous 3D neural spheroid system in 96 and 384-well format. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. 2 nM was calculated using SoftMax Pro Software. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. 과 같은 Parameter를 검사하여 평가할 수 있습니다. The Immunochemical Core includes approximately 2,600 square feet of space in the Joseph Building at Mayo Clinic Hospital, Saint Marys Campus. +44-118-944-8000. Molecular Devices SpectraMax i3x Multi-Mode Detection Platform. Here, we demonstrate the benefits of using the SpectraMax ® i3x Multi-Mode Microplate Reader with the SpectraMax ® MiniMax™ 300 Imaging Cytometer in combination with MetaMorph ® Microscopy Automation and Image Analysis Software for performing automated neurite outgrowth imaging and analysis. 3. ZERO BIAS - scores, article reviews, protocol conditions and moreThe SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and. SpectraMax i3x; SpectraMax iD3 / iD5; SpectraMax M Series; SoftMax Pro Software; CELLULAR IMAGING. All test measurements and calculations are handledPreconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. Tristar 5 Multimode Microplate Reader. Actual shake speed is based on the microplate format. Register to download your free eBook today. com. )wideand65. 2. Western blot assay 5 x 105 cells were seeded in 24-well plates in 500 μl growth medium and stimulated with novel compounds at 10μM. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. 0001 0. Multiple automated tests are provided including: • Linearity • Precision • Accuracy • Dynamic range • Alignment • Signal to noise • Signal to background • Filter checks. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. 00800 665 32860. Molecular Devices are known for both the reliability and the wide uses of their products. Setting iD5 i3x M5 Paradigm Measurement mode Fluorescence Polarization Fluorescence Polarization Fluorescence Polarization Fluorescence Polarization Excitation 485 nm monochromator 485 nm 485 nm 485 nmPage 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. 1 Microplate Data Acquisition & Analysis Software for Windows. Both readers demonstrated their ability to detect the HTRF signal from the terbium donor and red acceptor combination, and they could be used for saturation studies using the Tag-lite technology. The dual auto injector module. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. Description 1. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. If no sound is heard during initialization, contact technical support. 315 can't find zero order. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles). Biochrom New EX Read 400 Microplate Reader. . Bulk Kit. Benefits • User-upgradeable application modules including cellular imaging • Sensitivity across spectrum with Spectral Fusion™ Illumination • Expanded dynamic range with cooled PMT • Control and analytics provided by SoftMax® Pro Software SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves. 303 unable to cal dark current. With optimized reagents, option to operate the readers. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. A: percent GFP-positive cells calculated using the classification feature in the software. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. 0. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. 2-µL or 4-µL samples were pipetted onto a 64-well SpectraDrop slide in quadruplicate, following the plate map in Figure 2. . As shown in the results above, the DELFIA cell proliferation assay combined with the SpectraMax i3 reader gives researchers a very sensitive test for measuring cell proliferation. Results for SpectraMax i3x reader with NanoBRET Detection Module (blue) and on-board luminescence detection mode (orange). com Check our website for a current listing of worldwide distributors. 2 Parallel Line Analysis Conc log [ng/ML] RLU. Cell viability는 세포막 Integrity 또는. to View Figures. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. SpectraMax M5. . SeeThe SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. SpectraMax® L reader support; ü. 蛍光 発光 ELISA. The corrected absorbance is within 5% of the. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. As an aspirant Bioscience research with expertise in cell biology, immunology and bio instrumentation techniques, I bring a wealth of knowledge and expertise to the table. iD5 reader (the SpectraMax iD3 reader is also suitable for these assays, as it has the same onboard fluorescence performance as the SpectraMax iD5 reader), SpectraMax i3x, SpectraMax M4 or M5, and FilterMax F5 Multi-Mode Microplate Readers. . Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. Introduction. Open the catalog to page 1. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. Molecular Devices. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. We would like to show you a description here but the site won’t allow us. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. 2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. These robust readers have been placed in labs from Antarctica to the. ü; ü. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any application or budget. Materials and methodsSpectraMax i3x Multi-Mode Microplate Reader. The SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. Enzyme Activation Analysis;. pH-Xtra Glycolysis Assay - Agilent. Version 7. Supplier: Molecular Devices. 1 1 10 100 e e e e e e eSpectraMax Spectrophotometers for UV-Vis Absorbance Detection. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. 6% for the on-board optics (monochromators). One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. Background subtraction was not necessary. The solutions were thoroughly mixed and then incubated in the dark at 20°C for 2 h. Figure 4. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThis characterization of nanoparticle and its surface interactions can be detected using the SpectraMax i3x Multi-Mode Microplate Reader and the Spectral Optimization Wizard in SoftMax Pro Software. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploringfluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiterA microplate reader that can detect two or more applications is considered a multi-mode plate reader. . Eight different automated tests are provided: • Absorbance accuracy (linearity) • Precision (reproducibility) • Stray light • Wavelength accuracy • Wavelength repeatability SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Unlimited breadth of. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors; It allows you to explore cellular pathways and protein activation and expression in one system;In this application note, we describe how the SpectraMax ® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The plate was shaken and incubated in the dark for 10 minutes. Keywords: htrf spectramax detection cartridge, spectramax i3, spectramax paradigm system, htrf reagents, spectramax i3 and paradigm readers, HTRF assay, htrf. . Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. SpectraMax i3x, onboard luminescence 121. . 在这里,我们展示了如何使用SpectraMax®i3x和 SpectraMax®iD5多功能微孔板读板机应用HTRF技术进行 Gq-偶联受体的分析。G蛋白偶联受体的分析主要通过检测 cAMP和IP3这两条信号转导通路调控介导的细胞内钙离子水 平的升高。Cisbio公司的IP-One Gq试剂盒通过检. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesWith these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. china@moldev. Our absorbance plate readers feature our PathCheck Sensor. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. . Get a front row view of your cells. Serial number 33 900 01-3387. GFP expression imaged on the cytometer. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. SpectraMax i3x microplate reader and SoftMax Pro Software protocol. Danaher Inc spectramax i3x. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. . The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). Insert the snorkel into the bottle and then insert the snorkel into the snorkel clamp. SoftMax Pro User’s Manual v Contents Figures. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. Description 1. SpectraMax i3Instrumenttovisuallyinspectyoursampleandtoruncell-basedassaysat cellularorwhole-cellresolution. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. 2. 99. 100 fmol 1536-well (8 µL) 0. Find regional support. Spectranet customer portal. detection cartridge LUM96: 59. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Description 1. It allows you to explore cellular pathways and protein activation and expression in one system. . Note: Additional readers with similar performance are listed. i3x. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. Thetypeof luciferase(Renilla. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Spectramax I3x, supplied by Danaher Inc, used in various techniques. See Unpacking the SpectraMax Paradigm Instrument on page 1. Methods. Calcium. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Spectranet customer portal(SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published microplate reader control and microplate data analysis software package available to scientists. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. techsupport. Unplug the power cable from the back of the instrument. each new lot of plates, to ensure that the center of each well is read. com. Promega. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. Find regional support. Bioz Stars score: 98/100, based on 1 PubMed citations. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. Each heating element is independently. The information is below the horizontal line. TP medium was used as a blank and its fluorescence intensity was subtracted from that of mCherry or from chlorophyll auto-fluorescence intensity. Antigen Quantitation Protocol. Explore Multi-Mode Validation Plate that confirms instrument performance for spectramax i3, i3x, Paradigm system & FilterMax Reader. application modules • Multi-mode microplate reader and imaging system • Wide dynamic range • StainFree™ cell counting technology • Library of user-configurable applications • Advanced curve fitting and statistical analysis • Cell confluence and cell viabilitySoftMax® Pro 7. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. The assay can accurately test the toxicology of compounds as shown in Figure 1. The dual auto injector module. Reaction catalyzed by the firefly luciferase. TR-FRET ratios were calculated by dividing the RFU value for the 665-nm reading by the RFU value for the 616-nm reading, and the resulting numbers were multiplied by 1000. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. It allows you to explore cellular pathways and protein activation and expression in one system. 012 fmol 0. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置,请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整,以确保最佳 的检测灵敏度和动态范围。 数据分析 We would like to show you a description here but the site won’t allow us. The dynamic range of the assay is about 3. 1 software Download page. Example: [Drawer]. 314 low lamp level warning. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Determination of anti-inflammatory. The e ect of ASC-exosomes on the melanin levels inThe 70% methanolic extract of steamed root (100 µL) was added to 1 mL of diethylene glycol and 10 µL of sodium hydroxide (NaOH). This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. application modules • Multi-mode microplate reader and imaging system • Wide dynamic range • StainFree™ cell counting technology • Library of user-configurable applications • Advanced curve fitting and statistical analysis • Cell confluence and cell viability Introduction. 99. Published Dec 16, 2020 | Updated Oct 10, 2023. Contact Us Phone: +1. ATP (“flash” luminescence using . AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Bioz Stars score: 98/100, based on 1 PubMed citations. One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. 635. AlphaScreen. Expanded dynamic range The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube. Use the 3. 304 signal level saturation. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. iD5. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. Figure 1. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. Dilution series of HeLa cells transfected with firefly (red plot) and Renilla (blue plot) luciferases. 1321N1, a human astrocytoma cell line. which chemiluminescence was detected on a SpectraMax i3x reader (Molecular Devices) and analyzed us ing GraphPad. . SpectraMax i3x Register or Sign In. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. Register to download your free eBook today. SpectraMax i3x Multi-Mode Microplate Reader. 312 gain calibration failed. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. Request a Quote for This Setup. 58% for the detection module and 2. Download SoftMax® Pro 7. 7. Citation 2002). . The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Eliminate time-dependent substrate addition steps. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. 003 fmol 0. D 1 1. Quantiferon-TB Gold in Tube Kit. The SpectraMax i3x reader provides theSoftMaxProSoftwareFormulaReferenceGuide 8 5049093A SummaryFormulas Summariesconsistoffourparts: ASummaryname(whichisusedtorefertotheSummaryinotherformulas). 1 and above:. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置,请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整,以确保最佳 的检测灵敏度和动态范围。 数据分析Spectramax i3x fluorescence plate reader to measure fluo-rescence resonance energy transfer (FRET) signals from cell lines expressing CFP-YFP FRET sensors for glucose and ATP, as these sensors give large changes in FRET ratio in response to appropriate conditions, and are expressed in cells at high levels. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes With these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. SpectraMax i3x; SpectraMax iD3 / iD5; SpectraMax M Series; SoftMax Pro Software; CELLULAR IMAGING. Explore a wealth of applications with the future-ready SpectraMax i3x. 0. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013. ü; ü. Figure 4. Figure 3. Assay setup for a 384-well low-volume plate. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Description 1. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. QuickSync. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. For more information on the SpectraMax i3x Multi-Mode Microplate Reader System, please click here. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. Example: [Setup]. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. It has a cooled PMT, a no-stain cell analysis algorithm, and a field-upgradeable imaging cytometer. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. EMax Plus Microplate Reader. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular. Manufacturers try to design their instruments so they can be. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. 6% for the on-board optics (monochromators). Page 28 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 4. Overview of Western Blot Cartridge. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . 22mm 384 FilterMax™F3&F5Multi-ModeMicroplateReaders,SpectraMax®i3x Multi-ModeDetectionPlatform,SpectraMax®iD3&iD5Multi-Mode MicroplateReaders,andSpectraMax®Paradigm®Multi-Mode MicroplateReader 5301193 18mm 96 SpectraMaxi3x,SpectraMaxiD3,SpectraMaxiD5,SpectraMaxM. Unpacking and setting up the instrument includes the following procedures: • Unpacking the instrument and saving the original packaging. . 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. 302 low ref light. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. D 1 1. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology Measure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. SoftMax Pro Software enables users to quickly set up analysis of cell counts via a simple point and click user interface. . NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax i3x reader provides theThe SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway.